Journal: Nature methods

Article Title: Comprehensive comparative analysis of RNA sequencing methods for degraded or low input samples

doi: 10.1038/nmeth.2483

Figure Lengend Snippet: Shown are key metrics for RNase-H (orange), Ribo-Zero (pink) and total (black) libraries from pancreas and FFPE kidney RNA. ( a ) Percent of rRNA mapping reads; ( b ) Proportion of reads mapping to exons (solid), introns (hatched), and intergenic (white) regions; ( c ) mean coefficient of variation ( y axis) for the top 1,000 expressed transcripts in each library ( x axis); ( d ) Pearson correlation coefficient between each library and a control Total library.

Article Snippet: For human kidney FFPE tissue sections (Cybrdi), we isolated total RNA with the MasterPure RNA Purification Kit (Epicentre) following the manufacturer’s instructions with these modifications.

Techniques: Control

Journal: Cancer Science

Article Title: Radioimmunotherapy of human synovial sarcoma using a monoclonal antibody against FZD10

doi: 10.1111/j.1349-7006.2007.00701.x

Figure Lengend Snippet: Immunohistochemical staining analyses of synovial sarcoma and normal human tissue sections using MAb 92‐13; (a,b) synovial sarcoma tissue sections (c) colon, (d) heart, (e) lung, (f) liver, (g) kidney and (h) placenta. Original magnification ×100. Bar, 50 µm.

Article Snippet: For immunohistochemical staining analysis, 4‐µm sections of paraffin‐embedded normal adult human tissues (lung, heart, liver, kidney, colon and placenta) (BioChain, Hayward, CA, USA) and surgical synovial sarcoma specimens were deparaffinized and processed for antigen retrieval in Target Retrieval Solution (pH 9) (DAKO Cytomation, Carpinteria, CA, USA) at 125°C for 30 s. After treatment with peroxidase blocking reagent (DAKO Cytomation) for 10 min, followed by treatment with protein blocking serum‐free (DAKO Cytomation) for 30 min, slides were incubated with 10 µg/mL MAb 92‐13.

Techniques: Immunohistochemical staining, Staining